| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Venice |
A | Palmer | McDonald | Cernansky | Palmer | 8:13.32 | |
| 2 | Lakewood Ranch |
A | Leatt | Wagner | Johnson | Robinson | 8:18.00 | |
| 3 | Riverview (Sarasota) |
A | Holmes | Interiano | Taylor | 8:22.07 | FHSAA 4A District 8 | |
| 4 | North Port |
A | Sabo | Duque | Gallant | Storino | 8:25.98 | |
| 5 | Charlotte |
A | Davis | Ortiz | Sare | Lincoln-Velez | 8:26.00 | |
| 6 | Cardinal Mooney |
A | Garland | Hill | Kochanowicz | Shipe | 8:41.83 | FHSAA 2A Region 3 |
| 7 | Palmetto |
A | Ashton | Ashton | Rosales | Carlos | 8:46.86 | 8:46.86 Charlotte Invitational |
| 8 | Booker |
A | Mendoza | White | Duggan | Peterson | 9:27.83 | 9:27.83 Sarasota County Track and Field Championship Meet |
| 9 | Lemon Bay |
A | Skadal | Cobb | Roberts | Merritt | 9:32.96 | FHSAA 2A Region 3 |
| 10 | Manatee |
A | Bean | Shannon | Blake | Padilla | NT |