| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | St. Johns Country Day | A | DNS | 5:00.02 Clay County Championships |
| 2 | Duval Charter | A | DNS | |
| 3 | Florida Deaf and Blind | A | 4:37.20 | |
| 4 | Episcopal | A | DNS | FHSAA Class 2A/Para-Athlete Track & Field State Championship |
| 5 | University Christian | A | 4:30.26 | FHSAA 1A District 4 |
| 6 | Trinity Christian (Jax) | A | 4:51.83 | 4:51.83 Don Hall Invitational / St. John River Conference |
| 7 | Hilliard | A | DNS | 4:56.74 Yulee Meet %23 3 |
| 8 | Mandarin | A | NT |