| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | GUNTER, Bryce | FR | Interlachen | 2:33.15 | PALATKA JUNIOR SENIOR HIGH MEET #1 | 
| 2 | CUNNINGHAM, Sean | 7 | Providence | 2:33.34 | Providence Invitational 2021 | 
| 3 | TAUNTON, Noah | FR | Harvest Community | 2:35.95 | Bolles Mini Meet | 
| 4 | ANDERSON, JaiShawn | SR | Trinity Christian (Jax) | 2:37.59 | Danny Brown Invitational | 
| 5 | DUGAN, Zion | SR | St. Johns Country Day | 2:39.97 | FHSAA 1A District 4 | 
| 6 | ALLEN, Addison | 8 | Florida Deaf and Blind | 2:41.70 | Oak Hall Mini Meet #1 | 
| 7 | KOZLOWSKI, Harrison | 8 | Providence | 2:43.66 | St. Johns Country Day School - Meet 2 | 
| 8 | BREEN, Michael | SO | Christs Church Academy | 2:45.76 | Bolles Mini Meet | 
| 9 | BREEN, Johnny | 8 | Christs Church Academy | 2:47.52 | Bolles Mini Meet | 
| 10 | WINGATE, Mark | JR | Hilliard | 2:49.11 | FHSAA 1A District 4 | 
| 11 | BOGDEN, Dennis | SO | Interlachen | 2:50.73 | Ancient City Invitational | 
| 12 | TORRES, Noa | FR | Christs Church Academy | 2:56.64 | Bolles Mini Meet | 
| 13 | CENTENO, Roberto | SO | Florida Deaf and Blind | 3:06.92 | Matanzas HS Developmental | 
| 14 | BOULTON, Anthony | SR | Providence | NT | |
| 15 | CARD, Mason | SO | Hilliard | NT | |
| 16 | KIRTS, Mason | FR | Eagles View | NT | |
| 17 | OKEY, Jonathan | SO | Trinity Christian (Jax) | NT | |
| 18 | SCOTT, Jamorien | 8 | Florida Deaf and Blind | NT | |
| 19 | TRABITZSCH, Jon | JR | Duval Charter | NT | 
| Lane | Athlete | Yr. | Team | Seed | Note | 
|---|---|---|---|---|---|
| 1 | STRICKLIN, Nathan | SR | Harvest Community | 2:05.05 | 2:05.05 Bolles Last Chance Meet | 
| 2 | HOLEVA, Jake | JR | Christs Church Academy | 2:09.82 | FHSAA 1A District 4 | 
| 3 | LOFTIN, Claude | SO | Providence | 2:11.77 | FHSAA 1A District 4 | 
| 4 | MANSELLE, Cole | 8 | Eagles View | 2:12.13 | FHSAA 1A District 4 | 
| 5 | MACCLELLAN, Luca | SO | Providence | 2:15.00 | |
| 6 | WATKINS, Josiah | FR | Mandarin | 2:15.00 | |
| 7 | CHAPMAN, Tyler | FR | Trinity Christian (Jax) | 2:18.22 | FHSAA 1A District 4 | 
| 8 | TORREZ, Maurico | SR | Duval Charter | 2:20.42 | Mandarin Mustang Classic | 
| 9 | VASQUEZ, Leo | SR | Duval Charter | 2:20.86 | Mandarin Mustang Classic | 
| 10 | FREELAND, Connor | FR | Mandarin | 2:21.48 | Mandarin Mustang Developmental Meet 1 | 
| 11 | JACOBO, Noah | SO | Harvest Community | 2:21.92 | Danny Brown Invitational | 
| 12 | TOUPIN, Andrew | SR | Mandarin | 2:22.61 | Mandarin Mustang Classic | 
| 13 | WHITE, Walker | 8 | Providence | 2:22.87 | 2:22.87 Eagle Open | 
| 14 | YOUNG, Cameron | JR | Mandarin | 2:23.80 | Mandarin Mustang Classic | 
| 15 | BOST, Caleb | SO | Providence | 2:25.75 | Eagle Open | 
| 16 | FURTICK, Kendyel | FR | Mandarin | 2:26.00 | |
| 17 | POPP, Connor | SR | Providence | 2:29.67 | Providence Invitational 2021 | 
| 18 | OLAN, Caleb | FR | Trinity Christian (Jax) | 2:30.09 | Danny Brown Invitational | 
| 19 | SESSIONS, Jack | 8 | Providence | 2:31.43 | Eagle Open | 
| 20 | EDINGER, Hayden | 8 | Providence | NT |