| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | ROMERO, Erika | Bishop Verot | 2:41.26 | Tarpon Classic | 
| 2 | CIPOLLA, Kalee | First Baptist (Naples) | 2:44.16 | Tarpon Classic | 
| 3 | JOHNSON, Kasey | Evangelical Christian | 2:44.23 | Dunbar Tigers Guy Thomas Memorial Classic | 
| 4 | LEEF, Madeline | Southwest Florida Christian | 2:47.06 | First Baptist Academy (Naples) Invitational | 
| 5 | MCCLARAN, Kayley | Evangelical Christian | 2:50.07 | Calvary Christian Invitational | 
| 6 | HOBT, Isabella | Canterbury (FM) | 2:54.49 | Dunbar Tigers Guy Thomas Memorial Classic | 
| 7 | LONGO, Jeanette | Seacrest Country Day | 2:54.60 | GCAC MS Conference Championships | 
| 8 | COLLINS, Emma | St. John Neumann | 2:55.23 | Seacrest - SJN dual | 
| 9 | THOMAS, Ginger | Evangelical Christian | 2:56.10 | Dunbar Tigers Guy Thomas Memorial Classic | 
| 10 | STEPAN, Natalia | First Baptist (Naples) | 2:58.46 | McGarity's Annual Freshmen Sophomore Meet | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | BEAM, Katelyn | First Baptist (Naples) | 2:20.08 | Indian Rocks Big Invitational | 
| 2 | REITZ, Julia | Bishop Verot | 2:21.64 | Florida Relays | 
| 3 | BRANDS, Hayden | Canterbury (FM) | 2:30.74 | South Fort Myers Wolfpack Invitational | 
| 4 | SANTUCCI, Gabriella | Canterbury (FM) | 2:31.56 | Dunbar Tigers Guy Thomas Memorial Classic | 
| 5 | MANIBO, Hannah | Bishop Verot | 2:34.05 | Dunbar Tigers Guy Thomas Memorial Classic | 
| 6 | JANOPOULOS, Sloane | St. John Neumann | 2:34.25 | McGarity's Annual Freshmen Sophomore Meet | 
| 7 | GREEN, Abby | Southwest Florida Christian | 2:36.50 | First Baptist Academy (Naples) Invitational | 
| 8 | CHACHERE, Kate | St. John Neumann | 2:36.89 | Charlotte Invitational | 
| 9 | HAGENBUCKLE, Hanna | Community School (Naples) | 2:39.84 | Tarpon Classic | 
| 10 | HOBBS, Elizabeth | Community School (Naples) | 2:40.45 | 8th Annual Jim Smith Invitational |