| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 3 | CASEY, Bethany | First Baptist (Naples) | NT | 48.00 | 
| 4 | WALKER, Anna Leigh | St. John Neumann | 56.89 | Palmetto Ridge Invitational | 
| 5 | SEEBER, Kinsley | St. John Neumann | 57.86 | Titan Invite | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 2 | DARBY, Elianna | Southwest Florida Christian | 55.49 | First Baptist Academy (Naples) Invitational | 
| 3 | SANCHEZ, Allie | Community School (Naples) | 54.74 | McGarity's Annual Freshmen Sophomore Meet | 
| 4 | FEIT, Annabelle | Southwest Florida Christian | 53.77 | First Baptist Academy (Naples) Invitational | 
| 5 | ROMAN, Sofia | Bishop Verot | 53.94 | Tarpon Classic | 
| 6 | FABRIZI, Gianna | Evangelical Christian | 55.42 | Calvary Christian Invitational | 
| 7 | FIGUEROA, Hailey | Evangelical Christian | 56.01 | First Baptist Academy (Naples) Invitational | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | RIVERA-MUNIZ, Sofia | Community School (Naples) | 51.64 | First Baptist Academy (Naples) Invitational | 
| 2 | NEWMAN, Aniston | First Baptist (Naples) | 50.62 | First Baptist Academy (Naples) Invitational | 
| 3 | COGGINS, Grace | Southwest Florida Christian | 50.18 | Tarpon Classic | 
| 4 | CASEY, Hannah | First Baptist (Naples) | 47.39 | Tarpon Classic | 
| 5 | MARTIN-GONZALES, Leah | St. John Neumann | 49.92 | 8th Annual Jim Smith Invitational | 
| 6 | JULMEUS, Kristen | Evangelical Christian | 50.43 | First Baptist Academy (Naples) Invitational | 
| 7 | KENDALL, Lara | Canterbury (FM) | 51.43 | South Fort Myers Wolfpack Invitational | 
| 8 | BOLGIANO, Elise | Bishop Verot | 53.48 | Dunbar Tigers Guy Thomas Memorial Classic |