| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 3 | BRUEHL, Ethan | Bishop Verot | NT | NT | 
| 4 | CASSANO, Benjamin | Seacrest Country Day | 30.00 | First Baptist Academy (Naples) Invitational | 
| 5 | LOTHROP, Luke | St. John Neumann | NT | NT | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | HOLDERMAN, Gavin | St. John Neumann | 26.17 | Titan Invite | 
| 2 | FLOYD, Carson | St. John Neumann | 25.38 | McGarity's Annual Freshmen Sophomore Meet | 
| 3 | MALBRANCHE, Wesney | Evangelical Christian | 25.11 | South Fort Myers Wolfpack Invitational | 
| 4 | BAER, Jacob | Community School (Naples) | 24.86 | CSN Invitational Powered by UnderArmour | 
| 5 | KERSCHER, Grayson | Community School (Naples) | 25.02 | Palmetto Ridge Invitational | 
| 6 | FIDLER, Ben | Community School (Naples) | 25.32 | 8th Annual Jim Smith Invitational | 
| 7 | GORDON, Jayson | Canterbury (FM) | 25.66 | North Port Invite 2022 | 
| 8 | VERMA, Krish | Canterbury (FM) | 26.55 | North Port Invite 2022 | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | ROKER, Leroy | Canterbury (FM) | 24.54 | South Fort Myers Wolfpack Invitational | 
| 2 | WATTS, Trevontay | Bishop Verot | 24.18 | Bishop Verot - Viking Elite Classic 2022 | 
| 3 | VOTTA, Michael | First Baptist (Naples) | 23.80 | Spring Break Invitational at IMG | 
| 4 | PANZARELLA, Jacob | First Baptist (Naples) | 21.06 | Spring Break Invitational at IMG | 
| 5 | FABELA, Andrew | First Baptist (Naples) | 23.16 | First Baptist Academy (Naples) Invitational | 
| 6 | THOMAS, Ethan | Evangelical Christian | 23.98 | Calvary Christian Invitational | 
| 7 | GERGELY, Andrew | Evangelical Christian | 24.50 | Calvary Christian Invitational | 
| 8 | KEENAN, Mikhail | Bishop Verot | 24.80 | Charlotte Invitational |