| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | UNDERWOOD, Camyron | Auburndale |
23.57 | Lake Region Sprint Hurdle Night 2024 |
| 2 | JACKSON, Marlon | Lakeland |
23.49 | Dr. Phillips Panther Invitational |
| 3 | MCGOUGH, Jason | Auburndale |
23.47 | Polk County Freshman Sophomore Championships 2024 |
| 4 | SCOTT, Brayden | Winter Haven |
23.25 | Polk County Freshman Sophomore Championships 2024 |
| 5 | MAPP, Javon | George Jenkins |
23.31 | Berkeley Track and Field Invitational |
| 6 | DOBIE, DJ | Lakeland |
23.49 | Polk County Freshman Sophomore Championships 2024 |
| 7 | SENATUS, Welesly | Lake Region |
23.53 | Frostproof - The Ridge Open 2024 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | SLEDGE, Paul | Mulberry |
23.12 | Frostproof - The Ridge Open 2024 |
| 2 | TRUEDELL, AJ | Lakeland Christian |
23.01 | 2024 Lakeland City Championships |
| 3 | JEAN, Myrone | Lake Wales |
22.87 | Berkeley Track and Field Invitational |
| 4 | DOBIE, Ja'Darius | Lakeland |
22.73 | Dr. Phillips Panther Invitational |
| 5 | GALVA, Darrell | Bartow |
22.77 | George Jenkins - Bob English Invitational 2024 |
| 6 | MCCORMES, JaQuel | Winter Haven |
22.94 | Berkeley Track and Field Invitational |
| 7 | SEARIGHT, Trevon | McLaughlin | 23.08 | Polk County Freshman Sophomore Championships 2024 |
| 8 | BARSELOU, Daishawn | Kathleen |
23.24 | IMG Spring Break Invitational |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | JILES, Bryson | Auburndale |
22.39 | Blue Devil Invitational (Regional Qualifier) |
| 2 | HARDY, Derajah | Lakeland |
22.33 | Berkeley Track and Field Invitational |
| 3 | WILLIAMS, Terrance | Winter Haven |
22.09 | Berkeley Track and Field Invitational |
| 4 | DANIELS, Todd | Lake Wales |
21.96 | Berkeley Track and Field Invitational |
| 5 | TAYLOR, Janius | Bartow |
22.04 | Davenport - Bronco Invitational 2024 |
| 6 | TARVER, Jordan | Winter Haven |
22.17 | Berkeley Track and Field Invitational |
| 7 | ALLS, Jerry | Lake Wales |
22.38 | Berkeley Track and Field Invitational |
| 8 | BORDES, Joshua | Haines City |
22.51 | Frostproof - The Ridge Open 2024 |