| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | WALLIN, Angelina | McKeel |
13.44 | George Jenkins - Bob English Invitational 2024 |
| 3 | RUCKER, Mikiyah | Lakeland |
13.39 | Berkeley Track and Field Invitational |
| 4 | HENDERSON, Janyla | McLaughlin | 13.30 | Polk County Freshman Sophomore Championships 2024 |
| 5 | ALBRITTON, Savannah | George Jenkins |
13.37 | Berkeley Track and Field Invitational |
| 6 | CLEMENTE, Adriana | Davenport |
13.42 | George Jenkins - Bob English Invitational 2024 |
| 7 | NICOLSON, Raven | Lake Gibson |
13.47 | Blue Devil Invitational (Regional Qualifier) |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | TUNIS, Tanya | Kathleen |
13.24 | Kowboy Invitational |
| 2 | ALSTON, Justice | Lake Wales |
13.19 | Berkeley Track and Field Invitational |
| 3 | WALLER, Addison | Lakeland Christian |
13.16 | Blue Devil Invitational (Regional Qualifier) |
| 4 | ROGERS, Aiyana | Auburndale |
13.11 | Lake Region Sprint Hurdle Night 2024 |
| 5 | BRISBANE, Chelysei | Bartow |
13.13 | George Jenkins - Bob English Invitational 2024 |
| 6 | THOMAS, Amari | Haines City |
13.19 | Berkeley Track and Field Invitational |
| 7 | FORSETT, Keiera | Auburndale |
13.21 | Blue Devil Invitational (Regional Qualifier) |
| 8 | ASH, Dai'onna | Lake Gibson |
13.25 | Kowboy Invitational |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | KERLEGRAND, Adrianna | Winter Haven |
13.07 | Berkeley Track and Field Invitational |
| 2 | ADONTENG, Giuliana | Winter Haven |
12.89 | Berkeley Track and Field Invitational |
| 3 | ROSE, Jayana | George Jenkins |
12.85 | Berkeley Track and Field Invitational |
| 4 | PARRISH, Jayda | Auburndale |
12.82 | George Jenkins - Bob English Invitational 2024 |
| 5 | DENSON, Tristan | Bartow |
12.84 | Dr. Phillips Panther Invitational |
| 6 | HENDRIX, Lillyann | Bartow |
12.89 | George Jenkins - Bob English Invitational 2024 |
| 7 | ALGARIN, Naileany Encannacion | Bartow |
13.03 | Dr. Phillips Panther Invitational |
| 8 | HURST, Trinity | Haines City |
13.07 | Berkeley Track and Field Invitational |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | STREET, Geriah | George Jenkins |
12.59 | Berkeley Track and Field Invitational |
| 2 | ST. FLEUR, Nayelie | Lake Wales |
12.41 | Berkeley Track and Field Invitational |
| 3 | CONOLY, Ashari | Winter Haven |
12.22 | Berkeley Track and Field Invitational |
| 4 | MURRAY, Desirae | Winter Haven |
12.02 | Berkeley Track and Field Invitational |
| 5 | WHITEHURST, Azaria | Fort Meade |
12.13 | Berkeley Track and Field Invitational |
| 6 | WATKINS, Hailey | Santa Fe Catholic |
12.30 | Santa Fe Catholic season opener |
| 7 | GALLISHAW, Shaia | George Jenkins |
12.54 | Berkeley Track and Field Invitational |
| 8 | CANADAY, Zebrielle | Haines City |
12.71 | Berkeley Track and Field Invitational |