| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | WATKINS, Hailey | Santa Fe Catholic |
27.55 | Blue Devil Invitational (Regional Qualifier) |
| 3 | PAGE, McKenzie | Davenport |
27.39 | Polk County Freshman Sophomore Championships 2024 |
| 4 | ALGARIN, Naileany Encannacion | Bartow |
27.26 | Davenport - Bronco Invitational 2024 |
| 5 | MANAGO, Micaela | Lakeland |
27.30 | Berkeley Track and Field Invitational |
| 6 | JONES, Tajahlynn | Lakeland |
27.46 | Dr. Phillips Panther Invitational |
| 7 | SIMPSON, Issa | Lakeland Christian |
27.71 | Mustang Invitational |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | WILLIAMS, Michaelah | George Jenkins |
27.02 | George Jenkins - Bob English Invitational 2024 |
| 2 | ROGERS, Aiyana | Auburndale |
26.86 | Lake Region Sprint Hurdle Night 2024 |
| 3 | PARRISH, Jayda | Auburndale |
26.81 | Lake Region Sprint Hurdle Night 2024 |
| 4 | HENDRIX, Lillyann | Bartow |
26.60 | Blue Devil Invitational (Regional Qualifier) |
| 5 | ADONTENG, Giuliana | Winter Haven |
26.77 | Blue Devil Invitational (Regional Qualifier) |
| 6 | SOUTH, Tajhana | Lakeland |
26.86 | 2024 Lakeland City Championships |
| 7 | ROSE, Jayana | George Jenkins |
26.95 | Berkeley Track and Field Invitational |
| 8 | BULL, McKenzie | Bartow |
27.22 | Dr. Phillips Panther Invitational |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | KERLEGRAND, Adrianna | Winter Haven |
26.52 | Blue Devil Invitational (Regional Qualifier) |
| 2 | WHITEHURST, Azaria | Fort Meade |
25.91 | Berkeley Track and Field Invitational |
| 3 | STREET, Geriah | George Jenkins |
25.45 | Lake Region Sprint Hurdle Night 2024 |
| 4 | HENRY, Dashanique | Winter Haven |
24.93 | Blue Devil Invitational (Regional Qualifier) |
| 5 | MURRAY, Desirae | Winter Haven |
25.36 | Blue Devil Invitational (Regional Qualifier) |
| 6 | ST. FLEUR, Nayelie | Lake Wales |
25.74 | Berkeley Track and Field Invitational |
| 7 | GALLISHAW, Shaia | George Jenkins |
26.32 | Lake Region Sprint Hurdle Night 2024 |
| 8 | DENSON, Tristan | Bartow |
26.53 | Blue Devil Invitational (Regional Qualifier) |