| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | KIRKLAND, Ziyon | Auburndale |
54.29 | Blue Devil Invitational (Regional Qualifier) |
| 4 | DAVIS, Casey | Lake Wales |
54.13 | George Jenkins - Bob English Invitational 2024 |
| 5 | LILLARD, Tyrone | Lake Gibson |
54.24 | Winter Haven Classic |
| 6 | SEVERIT, Jase | Lake Gibson |
54.37 | Blue Devil Invitational (Regional Qualifier) |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | BOOKER, Brice | Lake Region |
54.11 | Polk County Freshman Sophomore Championships 2024 |
| 2 | GAMMAGE, Eddie | Frostproof |
53.79 | Davenport - Bronco Invitational 2024 |
| 3 | VALENTINE, Kaleef | Bartow |
53.75 | TFA - Royal Invitational 2024 (Regional Qualifier) |
| 4 | BOOKER, Devan | Lake Region |
53.51 | Blue Devil Invitational (Regional Qualifier) |
| 5 | ABRAM, Corion | Kathleen |
53.71 | George Jenkins - Bob English Invitational 2024 |
| 6 | DUNCAN, Johan | Auburndale |
53.76 | Lake Region Sprint Hurdle Night 2024 |
| 7 | DAVIS, Kenyarda | Tenoroc |
53.96 | Davenport - Bronco Invitational 2024 |
| 8 | LANGS, Robert | Lake Wales |
54.12 | Blue Devil Invitational (Regional Qualifier) |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | TRUEDELL, AJ | Lakeland Christian |
53.28 | Lake Region Sprint Hurdle Night 2024 |
| 2 | SENATUS, Welesly | Lake Region |
53.06 | Lake Region HS Distance Carnival 2024 |
| 3 | MCCORMES, JaQuel | Winter Haven |
52.94 | Blue Devil Invitational (Regional Qualifier) |
| 4 | GREEN, Malykih | Ridge Community |
52.37 | Lake Region Sprint Hurdle Night 2024 |
| 5 | PARKER, Sha'Ki | George Jenkins |
52.78 | Berkeley Track and Field Invitational |
| 6 | BATTLE, MARCUS | Lakeland |
53.00 | Blue Devil Invitational (Regional Qualifier) |
| 7 | KING, Charles | Lakeland |
53.14 | 2024 Lakeland City Championships |
| 8 | CASH, Anthony | George Jenkins |
53.46 | 2024 Lakeland City Championships |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MCCALLA, Jah'son | George Jenkins |
51.56 | 2024 Lakeland City Championships |
| 2 | KEARNEY, Michael | Winter Haven |
51.45 | Blue Devil Invitational (Regional Qualifier) |
| 3 | HARDY, Derajah | Lakeland |
50.80 | Dr. Phillips Panther Invitational |
| 4 | BRANTLEY, Carldayle | Lake Wales |
50.57 | Blue Devil Invitational (Regional Qualifier) |
| 5 | BUTLER, Rayshawn | Lake Gibson |
50.75 | Blue Devil Invitational (Regional Qualifier) |
| 6 | JEAN, Myrone | Lake Wales |
50.97 | Polk County Freshman Sophomore Championships 2024 |
| 7 | TARVER, Jordan | Winter Haven |
51.46 | Newsome Wolves Invitational 2024 |
| 8 | THOMAS, Armani | Tenoroc |
51.95 | Polk County Freshman Sophomore Championships 2024 |