| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 2 | 233 | BELL, Alex | SR | Crescent City | NT | |
| 3 | 55 | DORMEVIL, Jaden | FR | Matanzas | 1:10.00 | |
| 4 | 69 | LANE, Chandler | SR | Matanzas | 1:00.00 | |
| 5 | 57 | PHAN, Sophathan | JR | Matanzas | 1:01.72 | George Janan SC Invitational M |
| 6 | 210 | ALLENDER, Micah | 7 | Crescent City | NT | |
| 7 | 250 | WASHINGTON, Josiah | JR | Crescent City | NT |