| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | SIQUEIRA, Lidia | Sarasota Military Academy |
NT | |
| 2 | BOELLER, Amalia | Cardinal Mooney |
35.12 | JV Meet at Lakewood Ranch |
| 3 | ALCAZAR, Sol | Sarasota Military Academy |
33.57 | Hurricane Invitational Track and Field Meet |
| 4 | CHIDESTER, Kendal | Sarasota Christian | 32.28 | Larry Richardson Memorial Invitational |
| 5 | BELL, Lola | Bradenton Christian |
33.12 | Lakewood Ranch Invitational 2025 |
| 6 | BIRDSALL, Charlie | Out-of-Door |
33.74 | |
| 7 | REMIE, Valeria | Sarasota Military Academy |
41.35 | Hurricane Invitational Track and Field Meet |
| 8 | GIBSON, Avery | Bradenton Christian |
NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | REYNOLDS, Keyannah | Winthrop College Prep | 30.57 | Spring Break Invitational 2025 |
| 2 | LOVELACE, Maggie | Bradenton Christian |
30.23 | Eddie Starling Memorial Invitational |
| 3 | MCNEAL, Rayniyah | Sarasota Military Academy |
30.20 | Larry Richardson Memorial Invitational |
| 4 | BENZAN, Karlah | St. Stephens Episcopal |
29.82 | Saint Stephens Falcon Invite 2025 |
| 5 | PIERRE, Niya | Bradenton Christian |
30.00 | Eddie Starling Memorial Invitational |
| 6 | GINDOFF, Lexi | Out-of-Door |
30.20 | Saint Stephens MS MAC2 Invite 2025 |
| 7 | WYMAN, Dani | Out-of-Door |
30.55 | JV Meet at Lakewood Ranch |
| 8 | GRAHAM, Reagan | Out-of-Door |
32.10 | North Port Invitational 2025 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MALONI, Josie | Cardinal Mooney |
29.00 | jv meet |
| 2 | JOSEPH, Kimora | Winthrop College Prep | 28.70 | Spring Break Invitational 2025 |
| 3 | STEELE, Sophia | Cardinal Mooney |
26.70 | Lakewood Ranch Invitational 2025 |
| 4 | CARRIERA, Aniya | Winthrop College Prep | 25.54 | EB Tri-Meet 3/25/25 |
| 5 | EUGENE, Mikayla | Winthrop College Prep | 26.00 | Raven Invitational 2025 (Sanctioned) |
| 6 | TRESSELL, Bryhanna | Babcock | 28.66 | 65th Annual Edison Relays |
| 7 | CALOVICH, Katie | Cardinal Mooney |
28.90 | Lakewood Ranch Invitational 2025 |
| 8 | LAWRENCE, Hadley | St. Stephens Episcopal |
29.06 | Saint Stephens Falcon Invite 2025 |