| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | TRESSEL, Wyatt | Babcock | NT | |
| 2 | DITZEL, Josh | Bradenton Christian |
28.60 | John Tasopolos Memorial Invitational |
| 3 | DANGALAN, Ethan | Sarasota Christian | 27.12 | Saint Stephens Falcon Invite 2025 |
| 4 | EMMONS, Keywon | Sarasota Military Academy |
26.24 | Hurricane Invitational Track and Field Meet |
| 5 | PALACIOS, Daniel | Sarasota Military Academy |
27.04 | Pirate Booty Invitational |
| 6 | POST, James | Sarasota Christian | 27.61 | Larry Richardson Memorial Invitational |
| 7 | HEBERT, Ryan | Winthrop College Prep | NT | |
| 8 | HOFFMAN, Will | Babcock | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | BURGESS, Chance | Babcock | 25.96 | Palmetto Ridge Bear Classic |
| 2 | MORELAND, Caleb | Cardinal Mooney |
25.79 | Lakewood Ranch Invitational 2025 |
| 3 | FORD, John | Sarasota Military Academy |
25.34 | Larry Richardson Memorial Invitational |
| 4 | STINVIL, Snipes | Winthrop College Prep | 25.24 | 2025 Newsome Wolves Invitational |
| 5 | JOBE, Kamran | Out-of-Door |
25.30 | |
| 6 | PROUT, Brody | Babcock | 25.58 | Palmetto Ridge Bear Classic |
| 7 | ALEXIS, Taj | Sarasota Military Academy |
25.81 | Larry Richardson Memorial Invitational |
| 8 | CONLEY, Trent | Out-of-Door |
26.23 | JV Meet at Lakewood Ranch |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | CHINN, Ephraim | Out-of-Door |
24.89 | Saint Stephens Falcon Invite 2025 |
| 2 | BROWN, Trebreh | Winthrop College Prep | NT | |
| 3 | KIMBROUGH, Alijah | Winthrop College Prep | 23.86 | Spring Break Invitational 2025 |
| 4 | YOUNG, Kymistrii | Cardinal Mooney |
22.85 | Larry Richardson Memorial Invitational |
| 5 | JORDAN, Mason | Cardinal Mooney |
23.15 | Guy Thomas Memorial -Sponsored by Anderson Electric of SWFL |
| 6 | O'DANIEL, Bo | Cardinal Mooney |
24.44 | Pirate Booty Invitational |
| 7 | GAMBRELL, Camden | St. Stephens Episcopal |
24.56 | Sarasota Riverview - Ram Invite 2025 |
| 8 | CREWS, Nathan | Out-of-Door |
25.03 | North Port Invitational 2025 |