| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 3 | SOUKUP, Annie | Babcock | 26.23 | Lakewood Ranch Invitational 2025 |
| 4 | DEVERMANN, Aly | Babcock | 23.02 | Palmetto Ridge Bear Classic |
| 5 | BIRDSALL, Charlie | Out-of-Door |
23.40 | JV Meet at Lakewood Ranch |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | FERRY, Meikah | Babcock | 22.84 | Saint Stephens Falcon Invite 2025 |
| 3 | GINDOFF, Lexi | Out-of-Door |
20.87 | Saint Stephens MS MAC2 Invite 2025 |
| 4 | HARE, Eve | Cardinal Mooney |
18.53 | North Port Invitational 2025 |
| 5 | AUGUSTE, Djoudly | Cardinal Mooney |
20.20 | Guy Thomas Memorial -Sponsored by Anderson Electric of SWFL |
| 6 | BONFIG, Sophie | Babcock | 21.00 | Palmetto Ridge Bear Classic |