| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Out-of-Door | A | Iturralde | Dillingham | Douglas | Kints | 8:32.26 | IMG Academy Invitational |
| 2 | St. Stephens Episcopal | A | Vereb | Serra | Bick | Frano | 8:40.45 | FHSAA 1A District 11 |
| 3 | Cardinal Mooney | A | Yencik | Hassler | Michel | Sutthoff | 8:55.76 | FHSAA 1A District 11 |
| 4 | Riverview (Sarasota) | A | Quintero | Fenderson | Thomas | Holmes | 9:02.15 | Charlotte Invitational |
| 5 | Gibbs | A | Harris | Harris | Jenkins | Perez | DNS | St. Petersburg City Championship |
| 6 | Zephyrhills Christian | A | Brown | Blanc | Stanley | Yingling | DNS | Berkley Track and Field Invitational |
| 7 | Winthrop College Prep | A | Kimbrough | Betschner | Smith | Desrosiers | DNS | Mustang Invite |
| 8 | Hardee | A | Funderberg | Parker | Duncan | Nadaskay | NT |