| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 3 | GRAHAM, Destiny | Lockhart Middle School | 1:23.60 | |
| 4 | DAVIS, Charkevia | OCPS ACE | 1:15.09 | |
| 5 | SNODGRASS, Maya | Glenridge Middle School | 1:18.00 | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | JACKSON, G'Nyah | College Park Middle | 1:12.00 | |
| 2 | CLEMPSON, Rayelle | College Park Middle | 1:11.00 | |
| 3 | LANGSTON, Dalyia | OCPS ACE | 1:10.00 | |
| 4 | BRIDGES, Deriah | Howard Middle School | 1:09.80 | |
| 5 | LEON, Gabriella | Howard Middle School | 1:10.00 | |
| 6 | MCCLEMMENS, Kate | Maitland MS | 1:10.00 | |
| 7 | ROBINSON, Fanneciah | College Park Middle | 1:11.00 | |
| 8 | ALBU, Carly | Glenridge Middle School | 1:15.00 | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | ANGOV, Stella | Glenridge Middle School | 1:09.00 | |
| 2 | JUNOD, Prescott | Audubon Park School K-8 | 1:08.20 | |
| 3 | HALLIGAN, Maddie | Maitland MS | 1:04.00 | |
| 4 | REDDICKS, I'niya | Carver Middle School | 1:03.00 | |
| 5 | ALEXANDER, Sabine | Howard Middle School | 1:03.50 | |
| 6 | ARRIOLE, Janecia | Maitland MS | 1:06.00 | |
| 7 | AMBROISE, Lianni | Audubon Park School K-8 | 1:08.44 | |
| 8 | PARK, Madison | Audubon Park School K-8 | 1:09.19 |