| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note | 
|---|---|---|---|---|---|---|---|---|
| 2 | College Park Middle | A | Biglow | Johnson | Mosby | Jackson | 56.90 | |
| 3 | Glenridge Middle School | A | Phillips | Williams | Cineas | Barker | 55.23 | |
| 4 | Audubon Park School K-8 | A | Clark | Jackson | Junod | Barkett | 54.74 | |
| 5 | Maitland MS | A | Patterson | Simmons | Desir | Scanlon | 55.00 | |
| 6 | Howard Middle School | A | Roy | Stewart | Rowe | Chaney | DQ |