| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 3 | JENKINS, Hayden | Glenridge Middle School | NT | |
| 4 | O'ROURKE, Oliver | Glenridge Middle School | 1:08.00 | |
| 5 | MEJIA, Cesar | Lockhart Middle School | 1:12.35 | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | ORNELAS, Angel | Howard Middle School | 1:05.60 | |
| 2 | NEFF, Harrison | Maitland MS | 1:04.00 | |
| 3 | WHITE, Malik | Carver Middle School | 1:03.00 | |
| 4 | BIGLOW, James | College Park Middle | 1:03.00 | |
| 5 | BLANCHER, Zach | Glenridge Middle School | 1:03.00 | |
| 6 | LANNIGAN, Rion | Howard Middle School | 1:03.00 | |
| 7 | ZANDY, Luke | Maitland MS | 1:05.00 | |
| 8 | CASTO, Mateo | OCPS ACE | 1:06.00 | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | DELA CERNA, Gus | Howard Middle School | 1:02.00 | |
| 2 | MARCELLIN, Kabensley | Carver Middle School | 1:00.00 | |
| 3 | BARAKAT CRAINE, Luca | Audubon Park School K-8 | 59.00 | |
| 4 | TRISHAUN, Masai | Audubon Park School K-8 | 57.63 | |
| 5 | PRICE, Harrison | Maitland MS | 58.81 | |
| 6 | FOSTER, Dylan | College Park Middle | 59.25 | |
| 7 | DORRITY, Donnell | Audubon Park School K-8 | 1:01.60 | |
| 8 | WALKER, Tyree | College Park Middle | 1:02.00 |