| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | BROWN, Kendall | Nease | 27.67 |
| 2 | SHAVERS, Mikayla | Fleming Island | 27.64 |
| 3 | TAWDROUS, Rachael | Nease | 27.32 |
| 4 | WIGGINS, Mahaila | Fleming Island | 26.50 |
| 5 | GREEN, Victoria | Bartram Trail | 27.00 |
| 6 | BERKLEY, Kennedy | Fleming Island | 27.52 |
| 7 | BRAUGHTON, Taylor | Bartram Trail | 27.64 |
| 8 | HENLEY, Reese | Bartram Trail | 27.72 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | MUSA, Gabriella | Fleming Island | 29.00 |
| 2 | GANTHER, Peyton | Bartram Trail | 28.90 |
| 3 | OWENS, McKenna | Nease | 28.22 |
| 4 | MCCORMICK, Jayla | Bartram Trail | 27.90 |
| 5 | WILLIAMS, Isabella | Bartram Trail | 28.00 |
| 6 | O'CONNOR, Reece | Bartram Trail | 28.50 |
| 7 | NAYOCK, Alivia | Bartram Trail | 28.90 |
| 8 | JOHN, McKenzie | Bartram Trail | 29.00 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | LEVISTON, Autumn | Fleming Island | NT |
| 2 | HUNTER, Emma | Bartram Trail | 30.00 |
| 3 | PANDOLFI, Melina | Bartram Trail | 29.90 |
| 4 | REBEOR, Jacqueline | Bartram Trail | 29.40 |
| 5 | ABBOTT, Abree | Bartram Trail | 29.90 |
| 6 | SHAPIRO, Rilee | Bartram Trail | 30.00 |
| 7 | COSKEY, Anna | Bartram Trail | 30.00 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 3 | KNIGHT, Isabella | Nease | NT |
| 4 | PIERCE, Ariana | Fleming Island | NT |
| 5 | JENKINS, London | Fleming Island | NT |