| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | MARSHALL, Musthafa | Fleming Island | 24.04 |
| 2 | COOK, Brenden | Fleming Island | 23.11 |
| 3 | SINGLETON, Kaylib | Fleming Island | 22.52 |
| 4 | PATTERSON, Da'Von | Nease | 21.76 |
| 5 | SINGLETON, Samuel | Fleming Island | 22.00 |
| 6 | GILL, Dylan | Nease | 22.60 |
| 7 | WALKER, Danta | Bartram Trail | 23.56 |
| 8 | SCHULZE, Jacob | Bartram Trail | 24.10 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | LEWIS, Devante | Fleming Island | NT |
| 2 | VYAS, Lakshya | Bartram Trail | NT |
| 3 | DOUFIKAR, Ameer | Bartram Trail | 24.68 |
| 4 | EDWARDS, Matthew | Nease | 24.47 |
| 5 | CARVALHO, Guilherme | Bartram Trail | 24.60 |
| 6 | ROBINSON, Jalen | Bartram Trail | 25.30 |
| 7 | CHINMAY, Goswami | Fleming Island | NT |
| 8 | RANDALL, Ed | Fleming Island | NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 2 | MALATAK, Ethan | Bartram Trail | NT |
| 3 | DOMINECK, Darion | Fleming Island | NT |
| 4 | RODGERS, Jarius | Fleming Island | NT |
| 5 | GYAMFI, Anim | Bartram Trail | NT |
| 6 | SMITH, Jeremy | Bartram Trail | NT |
| 7 | TURNER, Royce | Bartram Trail | NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 2 | TREADWAY, Jackson | Bartram Trail | NT |
| 3 | THARPE, Gary | Bartram Trail | NT |
| 4 | JEAN, Sanjay | Fleming Island | NT |
| 5 | JACKSON, Dem'hir | Fleming Island | NT |
| 6 | BURNEY, Trace | Fleming Island | NT |