| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | OWENS, McKenna | Nease | 13.41 |
| 2 | MADDERN, Abbigael | Bartram Trail | 13.22 |
| 3 | BERKLEY, Kennedy | Fleming Island | 13.04 |
| 4 | JENKINS, London | Fleming Island | 12.80 |
| 5 | GANTHER, Peyton | Bartram Trail | 13.00 |
| 6 | MCCORMICK, Jayla | Bartram Trail | 13.20 |
| 7 | BROWN, Kendall | Nease | 13.27 |
| 8 | TAWDROUS, Rachael | Nease | 13.42 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | LEVISTON, Autumn | Fleming Island | 13.80 |
| 2 | NAYOCK, Alivia | Bartram Trail | 13.70 |
| 3 | KOSIEWSKA, Amelia | Bartram Trail | 13.60 |
| 4 | REICH, Daniela | Nease | 13.43 |
| 5 | BRAUGHTON, Taylor | Bartram Trail | 13.59 |
| 6 | LOPEZ ESTEBAN, Arianis | Nease | 13.64 |
| 7 | PIERCE, Ariana | Fleming Island | 13.80 |
| 8 | O'CONNOR, Reece | Bartram Trail | 13.80 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 2 | LANGELAND, Drue | Bartram Trail | 14.10 |
| 3 | JOHN, McKenzie | Bartram Trail | 14.00 |
| 4 | REBEOR, Jacqueline | Bartram Trail | 13.90 |
| 5 | MUSA, Gabriella | Fleming Island | 14.00 |
| 6 | MICKLER, Amelia | Bartram Trail | 14.10 |
| 7 | PANDOLFI, Melina | Bartram Trail | 14.30 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 3 | KNIGHT, Isabella | Nease | NT |
| 4 | HUNTER, Emma | Bartram Trail | 14.60 |
| 5 | COSKEY, Anna | Bartram Trail | 14.60 |