| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Plant |
A | 9:54.05 | |
| 2 | Alonso |
A | 10:05.08 | FHSAA Class 4A Track & Field State Championships |
| 3 | Newsome |
A | 10:15.00 | |
| 4 | Robinson |
A | DNS | FHSAA 2A Region 3 |
| 5 | Gaither |
A | 10:51.00 | |
| 6 | Brandon |
A | 10:59.65 | Spoto Quad Meet 4 |
| 7 | Palm Harbor University |
A | DNS | Pinellas County Athletic Conference (PCAC) |
| 8 | Wiregrass Ranch |
A | 11:11.11 | |
| 9 | Riverview |
A | DNS | 11:14.12 2023 Jack Rice Invitational |
| 10 | Tampa Bay Tech |
A | 11:18.00 | |
| 11 | Plant City |
A | DNS | |
| 12 | Hillsborough |
A | 12:27.00 | |
| 13 | Blake |
A | DNS | |
| 14 | Bloomingdale |
A | NT | |
| 15 | George Steinbrenner |
A | DNS | |
| 16 | Sickles |
A | NT | |
| 17 | Winthrop College Prep | A | NT |