| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 2 | Riverview |
A | DNS | |
| 3 | Newsome |
A | NT | |
| 4 | Lennard |
A | NT | |
| 5 | Sickles |
A | NT | |
| 6 | Spoto |
A | DNS | |
| 7 | Chamberlain |
A | DNS |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Blake |
A | 4:02.76 | 4:02.76 WC American Divisonals |
| 2 | Winthrop College Prep | A | 3:52.07 | Mustang Invite |
| 3 | Alonso |
A | 3:39.89 | 3:39.89 Raven Invitational 2023 |
| 4 | Middleton |
A | 3:36.23 | 3:36.23 WC National Division Meet |
| 5 | Wiregrass Ranch |
A | DNS | East Lake Early Bird Invitational |
| 6 | Palm Harbor University |
A | 3:49.83 | 3:49.83 East Lake Early Bird Invitational |
| 7 | Leto |
A | DQ | 3:55.27 2023 Manuel-Griffin-Fraundorfer Relays |
| 8 | Brandon |
A | DNS | 4:12.36 Newsome Quad 2/28 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Armwood |
A | 3:33.23 | |
| 2 | Plant City |
A | 3:30.00 | |
| 3 | Hillsborough |
A | 3:25.63 | FHSAA 3A Region 3 |
| 4 | Tampa Bay Tech |
A | DNF | FHSAA Class 4A Track & Field State Championships |
| 5 | Bloomingdale |
A | 3:24.55 | FHSAA Class 4A Track & Field State Championships |
| 6 | Sumner |
A | 3:30.00 | |
| 7 | Jefferson |
A | 3:33.17 | FHSAA 2A Region 3 |
| 8 | King |
A | DNS |