| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Freedom (Tampa) |
A | NT | |
| 2 | Winthrop College Prep | A | NT | |
| 3 | Bell Creek |
A | NT | |
| 4 | Alonso |
A | NT | |
| 5 | Brandon |
A | NT | |
| 6 | Spoto |
A | NT | |
| 7 | Leto |
A | NT |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Blake |
A | DNS | |
| 2 | Chamberlain |
A | 50.00 | |
| 3 | Gaither |
A | 45.83 | |
| 4 | Middleton |
A | 44.58 | FHSAA 3A Region 3 |
| 5 | Palm Harbor University |
A | 44.97 | Pinellas County Athletic Conference (PCAC) |
| 6 | Strawberry Crest |
A | 48.00 | |
| 7 | Riverview |
A | 1:05.30 | |
| 8 | Newsome |
A | NT |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | East Bay |
A | 44.17 | |
| 2 | Plant |
A | 44.00 | |
| 3 | Robinson |
A | 43.50 | |
| 4 | Sickles |
A | DNS | 43.26 WC American Divisonals |
| 5 | Wiregrass Ranch |
A | 43.46 | East Lake Early Bird Invitational |
| 6 | Durant |
A | 43.75 | |
| 7 | King |
A | 44.11 | 44.11 WC National Division Meet |
| 8 | Jefferson |
A | 44.53 | FHSAA 2A Region 3 |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Hillsborough |
A | DNS | |
| 2 | Sumner |
A | 42.70 | |
| 3 | Wharton |
A | 42.13 | 42.13 WC National Division Meet |
| 4 | Armwood |
A | DNF | FHSAA Class 4A Track & Field State Championships |
| 5 | Tampa Bay Tech |
A | 42.00 | 42.00 George Steinbrenner Invitational |
| 6 | Plant City |
A | 42.14 | |
| 7 | Lennard |
A | 42.82 | 42.82 2023 Manuel-Griffin-Fraundorfer Relays |
| 8 | Bloomingdale |
A | 42.94 | FHSAA Class 4A Track & Field State Championships |