| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Santa Fe Catholic |
B | NT | |
| 2 | Lake Region |
C | NT | |
| 3 | Auburndale |
B | NT | |
| 4 | Lake Minneola |
A | NT | |
| 5 | McLaughlin | A | NT | |
| 6 | Lake Minneola |
C | NT | |
| 7 | West Oaks |
A | DNS | |
| 8 | Mulberry |
A | NT |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Lake Region |
D | DNS | |
| 2 | Santa Fe Catholic |
A | DNS | 4:16.27 Mount Dora Christian Developmental meet %232 |
| 3 | Frostproof |
A | 4:00.00 | |
| 4 | George Jenkins |
D | 3:45.00 | |
| 5 | Tenoroc |
A | 3:47.32 | Winter Haven Classic |
| 6 | Lake Region |
B | 4:01.00 | |
| 7 | Discovery |
A | 4:17.19 | 4:17.19 Polk County Championship 2023 |
| 8 | Lakeland Christian |
B | NT |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | George Jenkins |
C | 3:40.00 | |
| 2 | Lakeland Christian |
A | 3:37.55 | 3:37.55 Jenkins Senior Night Meet |
| 3 | Ridge Community |
A | 3:31.00 | |
| 4 | Lake Minneola |
B | 3:26.52 | |
| 5 | Auburndale |
A | DNS | 3:27.68 Lake Region - Thunder Relays2023 |
| 6 | George Jenkins |
A | 3:32.00 | |
| 7 | George Jenkins |
B | 3:40.00 | |
| 8 | Poinciana |
A | 3:42.14 | 3:42.14 Poinciana Relays |