| Lane | Team | Squad | Seed | Note | 
|---|---|---|---|---|
| 1 |  Santa Fe Catholic | B | NT | |
| 2 |  Lake Region | C | NT | |
| 3 |  Auburndale | B | NT | |
| 4 |  Lake Minneola | A | NT | |
| 5 | McLaughlin | A | NT | |
| 6 |  Lake Minneola | C | NT | |
| 7 |  West Oaks | A | DNS | |
| 8 |  Mulberry | A | NT | 
| Lane | Team | Squad | Seed | Note | 
|---|---|---|---|---|
| 1 |  Lake Region | D | DNS | |
| 2 |  Santa Fe Catholic | A | DNS | 4:16.27 Mount Dora Christian Developmental meet %232 | 
| 3 |  Frostproof | A | 4:00.00 | |
| 4 |  George Jenkins | D | 3:45.00 | |
| 5 |  Tenoroc | A | 3:47.32 | Winter Haven Classic | 
| 6 |  Lake Region | B | 4:01.00 | |
| 7 |  Discovery | A | 4:17.19 | 4:17.19 Polk County Championship 2023 | 
| 8 |  Lakeland Christian | B | NT | 
| Lane | Team | Squad | Seed | Note | 
|---|---|---|---|---|
| 1 |  George Jenkins | C | 3:40.00 | |
| 2 |  Lakeland Christian | A | 3:37.55 | 3:37.55 Jenkins Senior Night Meet | 
| 3 |  Ridge Community | A | 3:31.00 | |
| 4 |  Lake Minneola | B | 3:26.52 | |
| 5 |  Auburndale | A | DNS | 3:27.68 Lake Region - Thunder Relays2023 | 
| 6 |  George Jenkins | A | 3:32.00 | |
| 7 |  George Jenkins | B | 3:40.00 | |
| 8 |  Poinciana | A | 3:42.14 | 3:42.14 Poinciana Relays |