| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 2 | PENA BRITO, Elaina | East River |
NT | NT |
| 3 | JOHN, Al'nisa | University (Orlando) |
36.93 | Lake Mary Invitational 2025 |
| 4 | COLEY, Talitha | University (Orlando) |
31.02 | East River Invitational |
| 5 | VIALA, Alexia | East River |
30.26 | Satellite High Reggie Johnson Invite |
| 6 | ARROYO, Gabriella | Colonial |
30.29 | Freedom Last Chance 2025 |
| 7 | CARTER-DUNSTON, Suhmer | Boone |
31.12 | Freedom Last Chance 2025 |
| 8 | PARATORE, Mia | Oak Ridge |
NT | NT |
| 9 | ROWE, Livia | Boone |
NT | NT |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MCNEILL-TORO, Santana | Innovation_Orlando | 30.20 | Harmony Invitational |
| 2 | HICKS, Aaliyah | Lake Nona |
29.45 | Freedom Last Chance 2025 |
| 3 | BROWN, Arielle | Oak Ridge |
29.36 | Oak Ridge - Pioneer Invitational 2025 |
| 4 | SUKUT, Catherine | Colonial |
29.26 | Mount Dora Christian Invitational #1 |
| 5 | MATHIS, Zomarria | Boone |
29.09 | Winter Park - Brian Jaeger Elite Classic 2025 |
| 6 | DAVIS, Aliyah | Freedom (Orlando) |
29.20 | Oak Ridge - Pioneer Invitational 2025 |
| 7 | HAMM, London | Lake Nona |
29.28 | Freedom Last Chance 2025 |
| 8 | TORRES, Natasha | Freedom (Orlando) |
29.41 | Freedom Last Chance 2025 |
| 9 | VASSOR, FriZariyah | University (Orlando) |
29.79 | Bears Invitational (Regional Qualifier) |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | FITZHERBERT, Sophia | Innovation_Orlando | 29.06 | Freedom Last Chance 2025 |
| 2 | CAMPOS, Ana | Winter Park |
28.48 | Winter Park - Wildcat Open 2025 |
| 3 | WILSON, Tatiana | East River |
28.37 | Cocoa Invitational |
| 4 | MATOS, Dayana | Freedom (Orlando) |
27.97 | Freedom Last Chance 2025 |
| 5 | BROWN, Alana | Oak Ridge |
27.68 | Oak Ridge - Pioneer Invitational 2025 |
| 6 | WILLIS, Jayla | Winter Park |
27.91 | Winter Park - Brian Jaeger Elite Classic 2025 |
| 7 | LONDONI, Helga | Innovation_Orlando | 28.19 | Oak Ridge - Pioneer Invitational 2025 |
| 8 | BROWN, Laci | Freedom (Orlando) |
28.44 | Oak Ridge - Pioneer Invitational 2025 |
| 9 | RODRIGUEZ, Giselly | Colonial |
28.75 | Mount Dora Christian Invitational #1 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | WINFIELD, Ava | East River |
27.45 | Cocoa Invitational |
| 2 | MULBAH, Emani | Timber Creek |
27.39 | 4th Annual North Florida/South Florida Challenge |
| 3 | DEKLE, Jalisa | Winter Park |
27.16 | ROYAL INVITATIONAL @ The First Academy 2025 |
| 4 | MOSS, Kamauria | Cypress Creek |
27.08 | Blue Devil Invitational (Regio |
| 5 | JONES-GRAHAM, Alaynia | Jones |
26.78 | Tiger Invitational |
| 6 | ARRIOLA, Janecia | Winter Park |
26.86 | Timber Creek Elite Classic 2025 |
| 7 | BROWN, Serenity | Jones |
27.11 | Bears Invitational (Regional Qualifier) |
| 8 | MOSS, Amaya | Lake Nona |
27.36 | ROYAL INVITATIONAL @ The First Academy 2025 |
| 9 | KIMMONS, Kaliyah | Lake Nona |
27.44 | Winter Park - Brian Jaeger Elite Classic 2025 |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | TURNER, Mariah | Innovation_Orlando | 26.68 | Oak Ridge - Pioneer Invitational 2025 |
| 2 | FERGUSON, Mya | Timber Creek |
26.59 | 4th Annual North Florida/South Florida Challenge |
| 3 | ROBBINS, Marchele | Boone |
26.43 | Freedom Last Chance 2025 |
| 4 | SHEFFIELD, Auriel | Jones |
25.16 | Ocoee Invitational |
| 5 | VALLE, Alexie | Timber Creek |
24.77 | Timber Creek Elite Classic 2025 |
| 6 | RODRIGUEZ, Alondra | Cypress Creek |
24.86 | Cypress Creek Early Season Meet 2025 |
| 7 | GILMER, Aniah | University (Orlando) |
26.14 | Lake Mary Invitational 2025 |
| 8 | NIX, Christian | Cypress Creek |
26.53 | Kowboy Invitational |
| 9 | THOMPSON, Tailin | Timber Creek |
26.60 | 2025 The Ellis Elite 16 Track and Field Invitational |