| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 2 | 228 | SPANN, Hazel | SO | Edgewater |
NT | |
| 3 | 390 | SIDDIQI, Sofia | SO | Winter Park |
NT | |
| 4 | 46 | MALAYKHAN, Crystal | JR | Lake Nona |
NT | |
| 5 | 273 | NOTARO, Elizabeth | SR | Edgewater |
NT | |
| 6 | 202 | NICHTER, Jillian | FR | Four Corners |
NT |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 166 | INGRAM, Kate | SO | Four Corners |
NT | |
| 2 | 77 | BECHERER, Annie | FR | Lake Nona |
NT | |
| 3 | 213 | SPENCER, Ava Grace | FR | Edgewater |
NT | |
| 4 | 203 | LOPEZ, Barbara | JR | Four Corners |
NT | |
| 5 | 135 | AWOSIKA, Obasindara | FR | First Academy (Orlando) |
NT | |
| 6 | 171 | KARNATH, Alyssa | 8 | Four Corners |
NT | |
| 7 | 79 | CHAO, Samantha | SR | Lake Nona |
NT | |
| 8 | 66 | MENDEZ, Andrea | FR | Lake Nona |
NT |
| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 65 | TABARES, Nicole | FR | Lake Nona |
NT | |
| 2 | 313 | GUITTON, Esme | SO | Winter Park |
NT | |
| 3 | 124 | BIRD, Makayla | JR | First Academy (Orlando) |
NT | |
| 4 | 153 | CLAYTOR, Charity | SO | First Academy (Orlando) |
1:40.67 | |
| 5 | 294 | BIGLOW, Jakiria | JR | Edgewater |
NT | |
| 6 | 47 | MOREIRA DE SOUZA, Isabella | FR | Lake Nona |
NT | |
| 7 | 44 | RAMOS RODRIGUES, Aljeandra | SO | Lake Nona |
NT | |
| 8 | 88 | ASHDOWN, Katie | FR | Lake Nona |
NT |