| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 3 | MCLEOD, Kyle | Boone | NT | |
| 4 | PRESSLEY, Jalen | Umatilla | NT | |
| 5 | MCGINNIS, Kehlil | University (Orlando) | NT | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | PEDRO, Jonathan | Lake Brantley | NT | |
| 2 | FLOWERS, Christian | Lake Brantley | NT | |
| 3 | PATTERSON, Julian | Lake Brantley | NT | |
| 4 | HAY, Jacob | Boone | NT | |
| 5 | SAFI, Evan | Lake Highland Prep | NT | |
| 6 | JURGENSEN, Bjorn | Bishop Moore | NT | |
| 7 | FELTON, Jonathon | Father Lopez | NT | |
| 8 | WALKER, Davion | University (Orlando) | NT | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | CASTRO, Andrew | Boone | NT | |
| 2 | OLEYAR, Cory | Windermere Prep | 26.17 | |
| 3 | HEATON, Christian | Umatilla | 25.53 | |
| 4 | SYLVESTER, Sy'Jahnee | University (Orlando) | 25.14 | Metro East Conference Championships | 
| 5 | SIMMONS, La'darius | Father Lopez | 25.24 | 2023 DELTONA HS SEASON OPENER | 
| 6 | AUGUSTIN, Fred | Orangewood Christian | 25.99 | FHSAA 1A District 7 | 
| 7 | WALKER, Charles | Orangewood Christian | 26.36 | Winter Park - Wildcat Open 2023 | 
| 8 | LOESER, Garrett | Umatilla | NT | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | GUMMER, Trenton | Lake Highland Prep | 24.34 | Bishop Moore - Carl Friedland Memorial Track Invite 2023 | 
| 2 | LASTER, Jadarious | West Oaks | 24.12 | Freedom Developmental Track Meet 2023 | 
| 3 | HERDEL, Ryan | Father Lopez | 23.57 | Winter Park - Wildcat Open 2023 | 
| 4 | CALCANO, Willy | Bishop Moore | 23.25 | Bishop Moore - Carl Friedland Memorial Track Invite 2023 | 
| 5 | COLLINS, Nicholas | Lake Highland Prep | 23.55 | Bishop Moore - Carl Friedland Memorial Track Invite 2023 | 
| 6 | COHEN, Marquis | West Oaks | 23.62 | Freedom Developmental Track Meet 2023 | 
| 7 | JUSME, William | Bishop Moore | 24.34 | FHSAA 3A District 6 | 
| 8 | BASS, Luca | Orangewood Christian | 25.02 | Winter Park - Wildcat Open 2023 |