| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | SHAH, Anya | Lake Highland Prep | 6:09.00 | Trinity Prep Relays 2022 | 
| 2 | HAGE, Eve | Bishop Moore | 6:11.57 | Trinity Prep Relays 2022 | 
| 3 | DE LA ROSA, Aileen | Boone | 6:26.16 | Winter Park - Wildcat Open 2023 | 
| 4 | MURPHY, Ava | Boone | 6:33.10 | Winter Park - Wildcat Open 2023 | 
| 5 | BOLSTEIN, Delaney | Lake Highland Prep | 6:33.12 | Carl Friedland Memorial | 
| 6 | RIVERA, Ariana | Boone | 6:35.42 | Brian Jaeger Elite Classic 2022 | 
| 7 | HANNA, Sophia | Lake Highland Prep | 6:38.10 | Carl Friedland Memorial | 
| 8 | DORCELY, Tessie | Bishop Moore | NT | |
| 9 | GUERRERO, Camila | Bishop Moore | NT | |
| 10 | GUZMAN, Margarita | Windermere Prep | NT | |
| 11 | HAGE, Elle | Bishop Moore | NT | |
| 12 | MADDEN, Kolbe | Lake Highland Prep | NT | |
| 13 | PATTERSON, Tara | Bishop Moore | NT | |
| 14 | POPAROD, Kennedy | Bishop Moore | NT | |
| 15 | RADFORD, Shannon | Bishop Moore | NT | |
| 16 | REYES, Amara | Bishop Moore | NT | |
| 17 | SMITH, Maya | Bishop Moore | NT | |
| 18 | YOUNG, Serena | Lake Highland Prep | NT |