| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 3 | SOLOMON, Natalia | University (Orlando) | NT | |
| 4 | GELLNER, Madison | Bishop Moore | NT | |
| 5 | BRUTUS, Herva | Umatilla | NT | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | BENNETT, Abigail | Orangewood Christian | NT | |
| 2 | LIVELY, Hailee | Umatilla | NT | |
| 3 | MADALINA, Gabby | Bishop Moore | NT | |
| 4 | BUTLER, Tasavia | West Oaks | 31.63 | |
| 5 | WALCOTT, Sydney | Windermere Prep | 37.43 | |
| 6 | SPEARS, Elise | Lake Brantley | NT | |
| 7 | ULYSSE, Lise | Lake Brantley | NT | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | EDOUARD, Emigrace | West Oaks | 30.00 | Freedom Developmental Track Meet 2023 | 
| 2 | SMITH, Alyssa | University (Orlando) | 29.48 | St. Cloud Invitational | 
| 3 | CHAMBERS, Jayla | Boone | 29.22 | Freedom High Open 2022 | 
| 4 | MILLER, Tabrea | West Oaks | 29.20 | |
| 5 | GREEN, Gabby | Father Lopez | 29.21 | 2023 DELTONA HS SEASON OPENER | 
| 6 | FRANCOIS, Naomie | Lake Brantley | 29.27 | Apopka HS Pre-Season Meet | 
| 7 | YOERGER, Alyssa | Bishop Moore | 29.73 | Mount Dora Christian Invitational Meet #5 | 
| 8 | SHORTER, Brylee | Father Lopez | 30.05 | 2023 DELTONA HS SEASON OPENER | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | SIMPSON, Madeline | Lake Highland Prep | 28.00 | FHSAA 2A District 7 | 
| 2 | MORRIS, Christina | University (Orlando) | 27.32 | Winter Park - Wildcat Open 2023 | 
| 3 | CAMPBELL, Trinitey | Umatilla | 27.03 | 27.03 South Sumter High School Raider Open | 
| 4 | GANZY, Alexis | Boone | 26.24 | |
| 5 | CANINE, Meryl | Lake Highland Prep | NT | |
| 6 | BRIDGER, Andreya | Father Lopez | 27.05 | 2023 DELTONA HS SEASON OPENER | 
| 7 | BACON, Kimaree | Lake Highland Prep | NT | |
| 8 | WILLIAMS, Mykel | Boone | 28.71 | Brian Jaeger Elite Classic 2022 |