| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 2 | DEL ROSARIO, Elina | Boone | NT | |
| 3 | CANDELARIO, Jeslyn | University (Orlando) | NT | |
| 4 | WHITE, Bailey | Geneva School | NT | |
| 5 | LEONEL, Amiyah | West Oaks | NT | |
| 6 | EL-KHORCHI, Sofia | Father Lopez | NT | |
| 7 | BROOKS, Ava | Lake Brantley | NT | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | MCCLARNON, Paige | Orangewood Christian | NT | |
| 2 | LIVELY, Hailee | Umatilla | NT | |
| 3 | ULYSSE, Lise | Lake Brantley | NT | |
| 4 | VOLLENWEIDER, Mayah | Geneva School | 1:21.09 | Seminole HS Open 2023 | 
| 5 | CHANCY, Stephanie | West Oaks | 1:25.81 | |
| 6 | EVERS, Mercedes | Orangewood Christian | NT | |
| 7 | FAJARDO, Jasmine | University (Orlando) | NT | |
| 8 | PARRADO, Isabella | Bishop Moore | NT | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | PATTERSON, Emma Jane | Bishop Moore | 1:16.41 | Trinity Prep - Citrus League Championship 2022 | 
| 2 | NEJAME, Alessandra | Lake Highland Prep | 1:15.36 | Windermere Meet of Champions Classic 2023 | 
| 3 | BEATY, Marlene | Father Lopez | 1:14.13 | 2023 DELTONA HS SEASON OPENER | 
| 4 | DOGHOR, Esther | University (Orlando) | 1:12.89 | St. Cloud Invitational | 
| 5 | PIERRE LOUIS, Leanna | Lake Brantley | 1:13.25 | Lake Mary Opening Classic 2023 | 
| 6 | BUTLER, Tasavia | West Oaks | 1:15.27 | |
| 7 | LEO, Erica | Father Lopez | 1:16.40 | 2023 DELTONA HS SEASON OPENER | 
| 8 | STEPHENS, Jamie | Umatilla | 1:18.79 | Villages Home Meet One 2023 | 
| Lane | Athlete | Team | Seed | Note | 
|---|---|---|---|---|
| 1 | STONE, Abby | Windermere Prep | 1:09.17 | Bishop Moore - Carl Friedland Memorial Track Invite 2023 | 
| 2 | GRANTHAM, Emily | Boone | 1:06.03 | Boone Invitational 2023 | 
| 3 | KRUEL, Peyton | Bishop Moore | 1:05.26 | Astronaut Invitational 2021 | 
| 4 | TROBVICH, Kate | Lake Highland Prep | NT | |
| 5 | OVERBY, Nia | Boone | 1:03.41 | Boone Invitational 2022 | 
| 6 | NOYES, Livia | Orangewood Christian | 1:05.89 | 1:05.89 Mount Dora Developmental Meet #2 | 
| 7 | MONTAS, Hillary | Lake Highland Prep | NT | |
| 8 | CARTHERS, Deniah | Umatilla | 1:09.83 | Lake- Sumter Championships |