| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Sarasota Military Academy |
A | NT | |
| 2 | Babcock | A | NT | |
| 3 | Imagine School |
A | 5:07.53 | Bobcat Relays |
| 4 | Out-of-Door |
A | 4:54.35 | 4:54.35 Sarasota County Track and Field Championship |
| 5 | Parrish |
A | 5:00.00 | |
| 6 | Southeast |
A | DNS | |
| 7 | Bradenton Christian |
A | NT |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Manatee |
A | DQ | 4:31.31 Manatee County Championship |
| 2 | North Port |
A | 4:23.60 | |
| 3 | Lakewood Ranch |
A | 4:21.79 | FHSAA 4A District 8 |
| 4 | Palmetto |
A | 4:19.50 | FHSAA 3A Region 3 |
| 5 | Sarasota |
A | 4:20.91 | Bobcat Relays |
| 6 | Cardinal Mooney |
A | 4:22.82 | 4:22.82 Bobcat Relays |
| 7 | Hardee |
A | 4:28.34 | FHSAA 2A Region 3 |
| 8 | Braden River |
A | 4:45.00 |