| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Babcock | A | NT | |
| 2 | Sarasota Christian | A | 4:02.00 | |
| 3 | Imagine School |
A | DNS | Bobcat Relays |
| 4 | St. Stephens Episcopal |
A | 3:40.15 | |
| 5 | Cardinal Mooney |
A | 3:46.12 | 3:46.12 Cardinal Mooney Invitational |
| 6 | Braden River |
A | 3:58.00 | |
| 7 | Sarasota Military Academy |
A | NT | |
| 8 | Southeast |
A | NT |
| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Sarasota |
A | 3:35.47 | Bobcat Relays |
| 2 | Lakewood Ranch |
A | 3:30.43 | 3:30.43 Manatee County Championship |
| 3 | Out-of-Door |
A | 3:28.49 | FHSAA State Track & Field Championships (1A) |
| 4 | Palmetto |
A | DNS | 3:24.56 Palmetto High School Invitational Meet |
| 5 | Manatee |
A | 3:26.79 | FHSAA 4A Region 2 |
| 6 | Parrish |
A | 3:29.62 | Bobcat Relays |
| 7 | Hardee |
A | 3:35.42 | FHSAA 2A Region 3 |
| 8 | North Port |
A | 3:39.55 |