| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | LOPEZ, Angela | City of Life Christian | NT |
| 2 | HARSHBARGER, Josie | Oviedo | NT |
| 3 | ORTIZ, Jennaya | City of Life Christian | NT |
| 4 | WILTZ, Noel | Lake Nona | NT |
| 5 | PUCKETT, Jazzmin | City of Life Christian | NT |
| 6 | PLATON, Olivia | Lake Nona | NT |
| 7 | ESTRADA, Lizeth | City of Life Christian | NT |
| 8 | MORRIS, Emma | Lake Nona | NT |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | VECCHIARELLI, Alexandra | Oviedo | NT |
| 2 | RAMIREZ, Kalena | City of Life Christian | NT |
| 3 | FLAY, Ashley | Lake Mary | 33.98 |
| 4 | SAPP, Taylor | Lake Mary | 32.08 |
| 5 | REESE, Karis | Lake Mary | 32.74 |
| 6 | BULLARD, Keniya | Lake Mary | 36.23 |
| 7 | ALLEY, Anthea | City of Life Christian | NT |
| 8 | RAMIREZ, Penelope | City of Life Christian | NT |