| Lane | Team | Squad | Seed | Note |
|---|---|---|---|---|
| 1 | Geneva School | A | 59.81 | Mount Dora Christian Developmental Meet #1 |
| 2 | Oviedo | A | 52.10 | |
| 3 | Lake Mary | A | 51.57 | Sanford Seminole Invitational |
| 4 | Rockledge | A | 47.88 | St. Cloud Track Invitational |
| 5 | Seminole (Sanford) | A | 49.58 | Sanford Seminole Invitational |
| 6 | Lake Howell | A | 51.80 | Sanford Seminole Invitational |
| 7 | Harmony | A | DQ | St. Cloud Track Invitational |
| 8 | City of Life Christian | A | NT |