| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 35 | STOJIC, Jaelin | FR | Lyman | 2:20.00 | |
| 2 | 130 | ESCOBAR, Jose | SR | Lake Howell | 2:23.89 | SAC Championship @ Lake Brantley 2021 |
| 3 | 25 | AKIN, Taner | FR | Lyman | 2:30.00 | |
| 4 | 18 | MARCHETTI, Logan | SO | Lyman | 2:35.80 | Lake Mary Invitational 2021 |
| 5 | 14 | DARWENT, Connor | FR | Lyman | 2:40.00 | |
| 6 | 24 | BISHOP, Jacob | SO | Lyman | NT | |
| 7 | 165 | ERDMANN-DUNLAP, David | SO | Hagerty | NT | |
| 8 | 345 | FATIZZI, Cameron | SO | International Community | NT | |
| 9 | 346 | HARSHBARGER, Wes | SO | International Community | NT | |
| 10 | 107 | LOWE, Kesean | JR | Lake Howell | NT | |
| 11 | 66 | MARCH, DeShawn | SO | Lyman | NT | |
| 12 | 121 | RAHMAN, Nicholas | FR | Lake Howell | NT | |
| 13 | 132 | SHANK, Alexander | FR | Lake Howell | NT | |
| 14 | 114 | SMITH, Hunter | FR | Lake Howell | NT | |
| 15 | 78 | VERDU, Michael | FR | Lake Howell | NT |