| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Bishop Moore |
A | Thrash | Jenkins | Fasen | Solby | 8:20.91 | ROYAL INVITATIONAL @ The First Academy 2025 |
| 2 | Menendez |
A | Hess | Buckley | Sivyer | Olivero | 9:03.23 | Palatka Track - Home Meet 1 |
| 3 | Windermere Prep |
A | Hollingshead | Pellegrini | Landeiro | Abdi | DNS | Storm Relays |
| 4 | Orangewood Christian |
A | Wild | Struyk | Bremer | Barsoum | DNS |