| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | South Walton |
A | Griffin | Ross | Madden | Prinz | 10:47.43 | Pine Forest Eagles Pre-Season Invitational |
| 2 | Geneva School |
A | Mosquera | Blanton | Gallo | Shepherd | 11:49.39 | Geneva Developmental Opener 2025 |
| 3 | Lake Highland Prep |
A | Chopra | Socarras | Kyriakou | Parekh | 11:56.86 | Sanford-Seminole Invitational 2025 |