| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lake Brantley |
A | Claudio | Gibbs | Edwards | Fuller | 9:31.41 | FHSAA 4A Region 1 |
| 2 | Spruce Creek |
A | Roy | Roy | Nugent | Hawkins | DNS | |
| 3 | Lake Mary |
A | Keller | Yuzzolin | Miskiv | King | 10:05.93 | FHSAA 4A District 3 |
| 4 | Flagler Palm Coast |
A | Tarsitano | Slaughter | De Young | Marcelus | 10:12.00 | |
| 5 | Oviedo |
A | Cupit | Schnoor | Norena | Calhoun | 10:15.00 | |
| 6 | Seminole (Sanford) |
A | Buhring | Heilman | Lautenceau | Snyder | 10:34.23 | FHSAA 4A District 3 |
| 7 | DeLand |
A | Bell | Bordeaux | Leonard | Lott | 10:46.50 | 5-Star Conference Championship (FHSAA Sanctioned Meet) |
| 8 | Geneva School |
A | Blanton | Gallo | Shepherd | Mosquera | 10:49.75 | FHSAA 1A Region 2 |
| 9 | Trinity Prep |
A | Del Vecchio | Hennebery | Gonzalez | Carlin | 10:55.74 | FHSAA 2A Region 2 |
| 10 | Hagerty |
A | Schwarzbauer | Keefer | Howell | Brewster | 11:00.11 |