| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Crooms |
A | CAINE | PARKES | PINKNEY | ADICHIRAYIL | DNS | |
| 2 | University (OC) |
A | Hunter | Hayes | Watson | Pastrana | 3:49.58 | 3:49.58 Deland HS Big D Relays 2024 |
| 3 | Hagerty |
A | Davis-Medina | Leon | Hernandez | Gandy | 3:38.00 | |
| 4 | Lake Highland Prep |
A | Allen | Henderson | Okaty | Dishman | DNS | 3:36.46 Olympia Titans Invitational |
| 5 | Oviedo |
A | Brunson | Hargrave | Febres | Menezes | 3:37.10 | FHSAA 4A District 3 |
| 6 | DeLand |
A | Caraballo | Cook | Grubbs | Mandell | 3:40.00 | |
| 7 | Trinity Prep |
A | Hawley | Sagotsky | Pini | Brunner | DNS |
| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Spruce Creek |
A | Korn | Campbell | Lucas | Maldonado | 3:34.22 | 2024 George Janan Spruce Creek Invitational |
| 2 | Flagler Palm Coast |
A | Haymon | Peterson | Najpaver | Jacobelli | 3:31.23 | |
| 3 | Lake Howell |
A | Patterson | Funnye' | Williams | Wilkerson | 3:28.86 | 3:28.86 Charlie Harris Relays |
| 4 | Lake Brantley |
A | McCarthy | Ramirez | Ayala | Francois | 3:23.61 | FHSAA 4A Region 1 |
| 5 | Lake Mary |
A | Jones | Holt | Williams, Jr | Kinnear | 3:28.65 | FHSAA 4A District 3 |
| 6 | Lyman |
A | Beason | McGowan | Harris | Sargent | 3:30.34 | FHSAA 3A District 6 |
| 7 | Geneva School |
A | Costar | Hendrix | Allen | Davis | 3:31.81 | FHSAA 1A Region 2 |
| 8 | Seminole (Sanford) |
A | Coissy | Loredo | Plummer | Rodriguez | 3:36.00 |