| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 459 | MASON, Scarlett | SO | Winter Springs |
11:44.63 | FHSAA 3A District 6 |
| 2 | 450 | RODRIGUEZ, Jolynn | SR | Winter Springs |
12:33.60 | Seminole Athletic Conference Championship 2023 |
| 3 | 423 | HOREL, Maya | SR | Winter Springs |
12:37.08 | Sanford - Seminole Invitational 2023 |
| 4 | 19 | BAKER, Isabel | SO | Orangewood Christian |
13:15.69 | Bishop Moore - Larry Kelly Invitational 2023 |
| 5 | 517 | SIMARD, Kara | JR | International Community |
13:19.88 | Lake Howell - Silverhawk Invitational 2023 |
| 6 | 1 | BAKER, Amelia | SO | Orangewood Christian |
13:36.96 | Bishop Moore - Larry Kelly Invitational 2023 |
| 7 | 244 | MILLER, Reilly | SR | Lake Brantley |
14:54.99 | Sanford - Seminole Invitational 2023 |
| 8 | 279 | AMIRKHOSRAVI, Leila | SO | Lake Brantley |
NT | NT |
| 9 | 30 | MCDONALD, Avery | SO | Orangewood Christian |
NT | NT |