| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 3 | South Lake |
A | White | Walker | Powell | Parker | 3:43.67 | |
| 4 | Circle Christian |
A | Houdashelt | LehmannSaba | Parker | Neeld | 3:28.21 | FHSAA Class 1A Track & Field State Championships |
| 5 | Lake Highland Prep |
A | Allen | Dishman | Okaty | Henderson | 3:30.00 | |
| 6 | Geneva School |
A | Davis | Hendrix | Costar | Liguori | 3:47.00 |