| Lane | # | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|---|
| 1 | 136 | SMITH, Za'Riyah | JR | Lake Buena Vista |
NM | |
| 2 | 88 | RENGGLI, Masha | JR | Freedom (Orlando) |
NM | |
| 3 | 589 | TURNER, Mariah | JR | Innovation_Orlando | NM | |
| 4 | 619 | GARNER, Ava | SO | Innovation_Orlando | 1.27m | West Orange - Dr. Baptiste Invitational 2025 |
| 5 | 657 | VALCIN DESSALINES, Rebekah | SO | Cypress Creek |
1.32m | Tiger Invitational |
| 6 | 812 | MILLIGAN, Nyah | SR | University (Orlando) |
1.57m | Bishop Moore - Carl Friedland Invite 2025 |