| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | JONES, Kimorah | Centennial |
NT | |
| 2 | FLOYD, Justyce | Fort Pierce Central |
32.41 | 32.41 South Fork Meet #2 2024 |
| 3 | ELIASSAINT, Edwina | Centennial |
30.44 | 30.44 SLC Championships 2024 |
| 4 | TOWNSEND, A'Yantee | Osceola (Kissimmee) |
29.94 | 29.94 St. Cloud Invitational |
| 5 | MARTINEZ-ROBLES, Laricette | Melbourne |
30.30 | 30.30 Cape Coast Conference Championship 2024 |
| 6 | COOK, Tiffany | Melbourne |
31.32 | 31.32 Cape Coast Conference Championship 2024 |
| 7 | LAZO, Jewelie | Osceola (Kissimmee) |
32.89 | 32.89 Coach Mac Monday Madness |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | MIZE, Aubrey | Melbourne |
29.37 | 29.37 Cape Coast Conference Championship 2024 |
| 2 | PLANTIN, Nylah | Centennial |
28.74 | 28.74 Jeff Fossen Invitational 2024 |
| 3 | DALY, Lindsey | Viera |
28.33 | 28.33 Cape Coast Conference Championship 2024 |
| 4 | RUTHERFORD, Paige | Viera |
28.24 | 28.24 Astronaut Developmental Meet 2024 |
| 5 | WEST, Aquanis | Fort Pierce Central |
28.30 | 28.30 28th Annual Warrior Invitational 2024 |
| 6 | PEREZ, Ariana | Martin County |
28.56 | 28.56 Jensen Beach / Chandler Wealth Invitational 2024 |
| 7 | COLLINS, Brianna | Harmony |
28.92 | 28.92 Coach Mac Monday Madness |
| 8 | WILLABUS-JONES, Ava | Osceola (Kissimmee) |
29.61 | 29.61 Kowboy Invitational |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | KOCHIS, Jayna | Martin County |
28.02 | 28.02 MCHS Tiger Invite 2024 |
| 2 | HAUSER, Sofia | St. Cloud |
27.33 | 27.33 Coach Mac Monday Madness |
| 3 | ABLORDEPPEY, Justyce | Viera |
27.12 | 27.12 Cape Coast Conference Championship 2024 |
| 4 | ANTENOR, C'aja | Martin County |
27.05 | 27.05 Martin County Championships 2024 |
| 5 | HOSKINS, Riyoko | Centennial |
27.07 | 27.07 SLC Championships 2024 |
| 6 | DOMASHINSKI, Sasha | Fort Pierce Central |
27.15 | 27.15 Bill Wilson Invitational 2024 |
| 7 | SCHENK, Imani | Melbourne |
27.51 | 27.51 Cape Coast Conference Championship 2024 |
| 8 | GILYARD, Gabriel | Harmony |
28.07 | 28.07 Kowboy Invitational |
| Lane | Athlete | Team | Seed | Note |
|---|---|---|---|---|
| 1 | LUNDY, Tatianna | Vero Beach |
26.94 | 26.94 Royal Palm Beach Invitational 2 |
| 2 | GIBSON, Azariah | Vero Beach |
26.72 | 26.72 Jensen Beach / Chandler Wealth Invitational 2024 |
| 3 | OLIVEIRA, Nicole | Harmony |
26.48 | 26.48 Kowboy Invitational |
| 4 | WILDER, La'Neal | Fort Pierce Central |
25.13 | 25.13 Bill Wilson Invitational 2024 |
| 5 | BRIGHT, Zanaya | Osceola (Kissimmee) |
26.27 | 26.27 Orange Belt Conference Championships |
| 6 | BENJAMIN, Kalani | Treasure Coast | 26.71 | 26.71 Bill Wilson Invitational 2024 |
| 7 | BROWN, Kaviya | Treasure Coast | 26.77 | 26.77 Jensen Beach / Chandler Wealth Invitational 2024 |
| 8 | BRODHEAD, Amaya | Viera |
27.01 | 27.01 Cape Coast Conference Championship 2024 |