| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | WRIGHT, Chandler | East Lake | 52.41 |
| 2 | LUDLAM, Ian | Alonso | 51.96 |
| 3 | ESCORT, DJay | Venice | 51.84 |
| 4 | THOMAS, Curtis | Bloomingdale | 50.87 |
| 5 | TURNBERGER, Cameron | North Port | 51.45 |
| 6 | HUNTER, Tahj | Sickles | 51.84 |
| 7 | LEVARITY, Shalamar | East Ridge | 52.40 |
| 8 | JEFFERS, Talik | Wharton | 52.94 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | LARKINS, Calvin | Riverview | 50.79 |
| 2 | BENDROSS, Gavin | George Jenkins | 50.39 |
| 3 | RUPE, Jordan | George Jenkins | 49.56 |
| 4 | GANT, Elijah | Lennard | 48.92 |
| 5 | MCCRAY, Willie | Lehigh | 49.14 |
| 6 | ROBBINS, Chase | Celebration | 49.97 |
| 7 | SAWYER, Jariyah | Plant City | 50.69 |
| 8 | LABOSSIERE, Nicard | North Port | 50.84 |