| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | NEWKIRK, Aellella | East Ridge | 17.63 |
| 2 | MURACK , Makena | Lakewood Ranch | 17.29 |
| 3 | SHELTON, Margaux | Plant | 16.49 |
| 4 | COX, Savannah | Lennard | 16.37 |
| 5 | LEGARE, Kareena | Venice | 16.45 |
| 6 | VILSAINT, Dalyah | George Jenkins | 17.08 |
| 7 | CRANE, Isabella | Venice | 17.33 |
| 8 | HENDERSON, Zakhia | East Ridge | 17.88 |
| Lane | Athlete | Team | Seed |
|---|---|---|---|
| 1 | CESAR, Janiya | Riverview | 16.19 |
| 2 | HILL, Micahela | East Lake | 15.83 |
| 3 | FLEMING, Kat | Wiregrass Ranch | 15.04 |
| 4 | CINCOTTA, Anna | East Lake | 14.57 |
| 5 | HUYKE, Helena | Celebration | 14.80 |
| 6 | GETER, Sanaa | Lehigh | 15.35 |
| 7 | MARTIN, Kaycee | Newsome | 15.91 |
| 8 | CLARK, Kylie | Newsome | 16.27 |