| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 2 | BOISROND, Jaden | SR | Olympia | NT | |
| 3 | MORENO, Timothy | JR | Olympia | 52.42 | 52.42 Metro West Championship |
| 4 | SURBER, Landis | JR | Freedom (Orlando) | 50.10 | 50.10 Lake Buena Vista Home Meet |
| 5 | PROPHET, James | SO | Cypress Creek | 51.71 | 51.71 St. Cloud Track Invitational |
| 6 | ROWLANDS, Tyler | SO | Cypress Creek | 53.28 | 53.28 Metro East Conference Championships |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WALRAVEN, Levi | SR | Freedom (Orlando) | 47.01 | 47.01 Metro East Conference Championships |
| 2 | MITCHELL, Ives | SO | Windermere | 46.69 | 46.69 Metro West Championship |
| 3 | DANIELS, Mason | SR | Oak Ridge | 46.14 | 46.14 Dr. Phillips Panther Invite |
| 4 | ROSIER, Anthony | SO | Windermere | 45.56 | 45.56 Metro West Championship |
| 5 | ALVARADO, Joshua | JR | Cypress Creek | 46.13 | 46.13 Metro East Conference Championships |
| 6 | SILVERA, RASHID | JR | Oak Ridge | 46.60 | 46.60 Metro East Conference Championships |
| 7 | TIMBERLAKE, Ashton | FR | Dr. Phillips | 46.86 | 46.86 Tiger Invitational |
| 8 | QUINTERO, KEVIN | JR | Oak Ridge | 49.25 | 49.25 Dr. Phillips Panther Invite |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | WESLEY, Samuel | JR | Boone | 44.35 | 44.35 Metro East Conference Championships |
| 2 | RATCLIFF JR., Kristopher | JR | Lake Nona | 43.90 | 43.90 Kowboy Developmental Meet 1 |
| 3 | LOPEZ, Ski | JR | Boone | 42.81 | 42.81 Dr. Phillips Panther Invite |
| 4 | HORTON, Roderick | SR | Boone | 39.75 | 39.75 Windermere Meet of Champions Classic 2022 |
| 5 | WASHINGTON, Syhion | JR | Dr. Phillips | 39.94 | 39.94 Tiger Invitational |
| 6 | JOSEPH, Amire | SR | Windermere | 43.02 | 43.02 Metro West Championship |
| 7 | VEGA, Darwin | SO | Tohopekaliga | 44.17 | 44.17 Don Hall Invitational |
| 8 | WARREN, Joshua | FR | Dr. Phillips | 45.17 | 45.17 Dr. Phillips Panther Invite |