| Lane | Team | Squad | Athlete 1 | Athlete 2 | Athlete 3 | Athlete 4 | Seed | Note |
|---|---|---|---|---|---|---|---|---|
| 1 | Lake Wales | A | Morales | Cotto | Updike | Tellis | DNF | FHSAA 3A District 7 |
| 2 | Sunlake | A | Whitfield | Pena | Meyer | Robbins | 8:20.21 | FHSAA 3A District 6 |
| 3 | Chamberlain | A | Davis | Pettway | Ortigoza-Rodriguez | Fogg | 8:26.62 | FHSAA 3A District 8 |
| 4 | Middleton | A | Hodges | Evina-Ze | Tompkins | Harris | 8:30.62 | FHSAA 3A District 8 |
| 5 | Land O'Lakes | A | Levesque | Castillo | Martin | Sallengs | 8:30.86 | FHSAA 3A District 6 |
| 6 | Jesuit (Tampa) | A | Stevens | Tan | Brazzeal | Addison | 8:35.03 | FHSAA 3A District 8 |
| 7 | Lake Minneola | A | Bradshaw | Bradshaw | Reed | Carrillo | 8:43.54 | FHSAA 3A District 5 |
| 8 | Lake Region | A | Skipper | Toussaint | Collins | Jones | 8:45.01 | FHSAA 3A District 7 |
| 9 | Harmony | A | Romer | Jean | Sanchez | Yi | 8:45.74 | FHSAA 3A District 5 |
| 10 | Zephyrhills | A | Clemens | Day | Gomez | Poe | 8:49.45 | FHSAA 3A District 7 |
| 11 | South Lake | A | Hodge | Stansfield | Hovius | Richardson | 8:52.34 | FHSAA 3A District 5 |
| 12 | Robinson | A | Mccray | Yoder | Difo | Hearing | 8:56.26 | FHSAA 3A District 8 |
| 13 | Edgewater | A | Wallace | Fenelon | Wallace | Woodson | 9:04.02 | FHSAA 3A District 5 |
| 14 | Lecanto | A | Sullivan | Wilsek | Holzer | Nkwocha | 9:17.70 | FHSAA 3A District 6 |
| 15 | Brandon | A | Amador | Vasquez | Vickers | Bernard | 9:30.31 | FHSAA 3A District 7 |
| 16 | Springstead | A | Rosario | Brittain | Danchise | Kader | 9:59.79 | FHSAA 3A District 6 |