| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | CLOWER, Jakavious | SO | Zephyrhills | 44.32 | FHSAA 3A District 7 |
| 2 | JUNG, Daniel | SR | Jesuit (Tampa) | 43.90 | FHSAA 3A District 8 |
| 3 | CHERANCY, Watson | JR | Harmony | 43.36 | FHSAA 3A District 5 |
| 4 | JACKSON, Quincy | SR | Middleton | 42.74 | FHSAA 3A District 8 |
| 5 | SULLIVAN, Joseph | JR | Lecanto | 43.00 | FHSAA 3A District 6 |
| 6 | LAARI, Erik | JR | Sunlake | 43.69 | FHSAA 3A District 6 |
| 7 | TUCKER, Marcell | SO | Zephyrhills | 43.94 | FHSAA 3A District 7 |
| 8 | ALVARADO-BORJAS, Gabriel | SO | Jesuit (Tampa) | 44.74 | FHSAA 3A District 8 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | SALAAM, Delvin | SR | Lake Minneola | 42.31 | FHSAA 3A District 5 |
| 2 | TUYO, Alex | SO | Harmony | 41.83 | FHSAA 3A District 5 |
| 3 | SAINTVALLE, Chris | SO | Mitchell | 41.53 | FHSAA 3A District 6 |
| 4 | NEAL, Maguire | JR | Zephyrhills | 40.13 | FHSAA 3A District 7 |
| 5 | JACKSON, Jamaul | SR | Brandon | 40.71 | FHSAA 3A District 7 |
| 6 | ADCOCK, Tyler | SR | Land O'Lakes | 41.60 | FHSAA 3A District 6 |
| 7 | WEBB, Kylen | SO | Sumner | 42.09 | FHSAA 3A District 8 |
| 8 | HENRY, Rickkem | SR | Gateway (Kissimmee) | 42.73 | FHSAA 3A District 5 |