| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | RANKIN, Adyana | SR | Kathleen | 53.90 | FHSAA 3A District 7 |
| 2 | WOODALL, Shawna | SR | Hillsborough | 53.25 | FHSAA 3A District 8 |
| 3 | GUNABE-MORGAN, Genesis | FR | East River | 52.18 | FHSAA 3A District 5 |
| 4 | COLE, Jada | SR | Land O'Lakes | 50.99 | FHSAA 3A District 6 |
| 5 | GROPPE, Kayla | JR | Mitchell | 51.08 | FHSAA 3A District 6 |
| 6 | MORRIS, Victoria | SR | Auburndale | 52.47 | FHSAA 3A District 7 |
| 7 | WYCKOFF, Emaleth | SO | East River | 53.66 | FHSAA 3A District 5 |
| 8 | REYES, Anayah | FR | Robinson | 54.81 | FHSAA 3A District 8 |
| Lane | Athlete | Yr. | Team | Seed | Note |
|---|---|---|---|---|---|
| 1 | LEWIN, Arianna | JR | Kathleen | 49.76 | FHSAA 3A District 7 |
| 2 | SMITH, Syndi | SR | Spoto | 48.66 | FHSAA 3A District 8 |
| 3 | MASSILION, Sarah | JR | Middleton | 47.66 | FHSAA 3A District 8 |
| 4 | D'ANNA, Celine | SR | Lecanto | 46.61 | FHSAA 3A District 6 |
| 5 | THOMAS, Tyra | SR | Auburndale | 47.09 | FHSAA 3A District 7 |
| 6 | DOLL, Alexis | SR | Lake Minneola | 48.65 | FHSAA 3A District 5 |
| 7 | MOORE, Ida Rose | SR | Gulf | 49.28 | FHSAA 3A District 6 |
| 8 | MILLER, aysha | SR | Jones | 49.88 | FHSAA 3A District 5 |